Transplantation of Allogeneic PW1pos/Pax7neg Interstitial Cells Enhance Endogenous Repair of Injured Porcine Skeletal Muscle

• Main Authors: Fiona C. Lewis, BSc, PhD, Beverley J. Cottle, BSc, PhD, Victoria Shone, BSc, MSc, PhD, Giovanna Marazzi, MD, David Sassoon, PhD, Cheyenne C.S. Tseng, MD, Patricia Y.W. Dankers, PhD, Steven A.J. Chamuleau, MD, PhD, Bernardo Nadal-Ginard, MD, PhD, Georgina M. Ellison-Hughes, BSc, PhD
• Format: Article
• Language: English
• Published: Elsevier 2017-12-01
• Series: JACC: Basic to Translational Science
• Subjects: allogeneic progenitor cells; growth factors; PICs; porcine preclinical model; regeneration; skeletal muscle
• Online Access: http://www.sciencedirect.com/science/article/pii/S2452302X17302255
• ISSN: 2452-302X

Skeletal muscle-derived PW1pos/Pax7neg interstitial cells (PICs) express and secrete a multitude of proregenerative growth factors and cytokines. Utilizing a porcine preclinical skeletal muscle injury model, delivery of allogeneic porcine PICs (pPICs) significantly improved and accelerated myofiber regeneration and neocapillarization, compared with saline vehicle control-treated muscles. Allogeneic pPICs did not contribute to new myofibers or capillaries and were eliminated by the host immune system. In conclusion, allogeneic pPIC transplantation stimulated the endogenous stem cell pool to bring about enhanced autologous skeletal muscle repair and regeneration. This allogeneic cell approach is considered a cost-effective, easy to apply, and readily available regenerative therapeutic strategy.