Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways.
As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adapt...
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2011-11-01
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Series: | PLoS Genetics |
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doaj-267b6741cba6464c9b09fc1d98cbfecc2020-11-25T00:02:54ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042011-11-01711e100237710.1371/journal.pgen.1002377Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways.Mohan BabuJ Javier Díaz-MejíaJames VlasblomAlla GagarinovaSadhna PhanseChris GrahamFouad YousifHuiming DingXuejian XiongAnaies Nazarians-ArmavilMd AlamgirMehrab AliOxana PogoutseAsaf Pe'erRoland ArnoldMagali MichautJohn ParkinsonAshkan GolshaniChris WhitfieldShoshana J WodakGabriel Moreno-HagelsiebJack F GreenblattAndrew EmiliAs the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among > 235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target.http://europepmc.org/articles/PMC3219608?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohan Babu J Javier Díaz-Mejía James Vlasblom Alla Gagarinova Sadhna Phanse Chris Graham Fouad Yousif Huiming Ding Xuejian Xiong Anaies Nazarians-Armavil Md Alamgir Mehrab Ali Oxana Pogoutse Asaf Pe'er Roland Arnold Magali Michaut John Parkinson Ashkan Golshani Chris Whitfield Shoshana J Wodak Gabriel Moreno-Hagelsieb Jack F Greenblatt Andrew Emili |
spellingShingle |
Mohan Babu J Javier Díaz-Mejía James Vlasblom Alla Gagarinova Sadhna Phanse Chris Graham Fouad Yousif Huiming Ding Xuejian Xiong Anaies Nazarians-Armavil Md Alamgir Mehrab Ali Oxana Pogoutse Asaf Pe'er Roland Arnold Magali Michaut John Parkinson Ashkan Golshani Chris Whitfield Shoshana J Wodak Gabriel Moreno-Hagelsieb Jack F Greenblatt Andrew Emili Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways. PLoS Genetics |
author_facet |
Mohan Babu J Javier Díaz-Mejía James Vlasblom Alla Gagarinova Sadhna Phanse Chris Graham Fouad Yousif Huiming Ding Xuejian Xiong Anaies Nazarians-Armavil Md Alamgir Mehrab Ali Oxana Pogoutse Asaf Pe'er Roland Arnold Magali Michaut John Parkinson Ashkan Golshani Chris Whitfield Shoshana J Wodak Gabriel Moreno-Hagelsieb Jack F Greenblatt Andrew Emili |
author_sort |
Mohan Babu |
title |
Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways. |
title_short |
Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways. |
title_full |
Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways. |
title_fullStr |
Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways. |
title_full_unstemmed |
Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways. |
title_sort |
genetic interaction maps in escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2011-11-01 |
description |
As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among > 235,000 digenic mutant combinations tested reveal unexpected condition-specific functional crosstalk and genetic backup mechanisms that ensure stress-resistant envelope assembly and maintenance. These networks also provide insights into the global systems connectivity and dynamic functional reorganization of a universal bacterial structure that is both broadly conserved among eubacteria (including pathogens) and an important target. |
url |
http://europepmc.org/articles/PMC3219608?pdf=render |
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