The placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza.
<h4>Background</h4>Pregnancy increases susceptibility to influenza. The placenta releases an immunosuppressive endogenous retroviral protein syncytin-1. We hypothesised that exposure of peripheral monocytes (PBMCs) to syncytin-1 would impair responses to H1N1pdm09 influenza.<h4>Met...
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doaj-26b4a51809fc4f2293c6ac784c1acb9e2021-03-04T08:23:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e011862910.1371/journal.pone.0118629The placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza.Jorge M TolosaKristy S ParsonsPhilip M HansbroRoger SmithPeter A B Wark<h4>Background</h4>Pregnancy increases susceptibility to influenza. The placenta releases an immunosuppressive endogenous retroviral protein syncytin-1. We hypothesised that exposure of peripheral monocytes (PBMCs) to syncytin-1 would impair responses to H1N1pdm09 influenza.<h4>Methods and findings</h4>Recombinant syncytin-1 was produced. PBMCs from non-pregnant women (n=10) were exposed to H1N1pdm09 in the presence and absence of syncytin-1 and compared to responses of PBMCs from pregnant women (n=12). PBMCs were characterised using flow cytometry, release of interferon (IFN)-α, IFN-λ, IFN-γ, IL-10, IL-2, IL-6 and IL-1β were measured by cytometric bead array or ELISA. Exposure of PBMCs to H1N1pdm09 resulted in the release of IFN-α, (14,787 pg/mL, 95% CI 7311-22,264 pg/mL) IFN-λ (1486 pg/mL, 95% CI 756-2216 pg/mL) and IFN-γ (852 pg/mL, 95% CI 193-1511 pg/mL) after 48 hours. This was significantly impaired in pregnant women (IFN-α; p<0.0001 and IFN-λ; p<0.001). Furthermore, in the presence of syncytin-1, PBMCs demonstrated marked reductions in IFN-α and IFN-λ, while enhanced release of IL-10 as well as IL-6 and IL-1β.<h4>Conclusions</h4>Our data indicates that a placental derived protein, syncytin-1 may be responsible for the heightened vulnerability of pregnant women to influenza.https://doi.org/10.1371/journal.pone.0118629 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jorge M Tolosa Kristy S Parsons Philip M Hansbro Roger Smith Peter A B Wark |
spellingShingle |
Jorge M Tolosa Kristy S Parsons Philip M Hansbro Roger Smith Peter A B Wark The placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza. PLoS ONE |
author_facet |
Jorge M Tolosa Kristy S Parsons Philip M Hansbro Roger Smith Peter A B Wark |
author_sort |
Jorge M Tolosa |
title |
The placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza. |
title_short |
The placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza. |
title_full |
The placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza. |
title_fullStr |
The placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza. |
title_full_unstemmed |
The placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza. |
title_sort |
placental protein syncytin-1 impairs antiviral responses and exaggerates inflammatory responses to influenza. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
<h4>Background</h4>Pregnancy increases susceptibility to influenza. The placenta releases an immunosuppressive endogenous retroviral protein syncytin-1. We hypothesised that exposure of peripheral monocytes (PBMCs) to syncytin-1 would impair responses to H1N1pdm09 influenza.<h4>Methods and findings</h4>Recombinant syncytin-1 was produced. PBMCs from non-pregnant women (n=10) were exposed to H1N1pdm09 in the presence and absence of syncytin-1 and compared to responses of PBMCs from pregnant women (n=12). PBMCs were characterised using flow cytometry, release of interferon (IFN)-α, IFN-λ, IFN-γ, IL-10, IL-2, IL-6 and IL-1β were measured by cytometric bead array or ELISA. Exposure of PBMCs to H1N1pdm09 resulted in the release of IFN-α, (14,787 pg/mL, 95% CI 7311-22,264 pg/mL) IFN-λ (1486 pg/mL, 95% CI 756-2216 pg/mL) and IFN-γ (852 pg/mL, 95% CI 193-1511 pg/mL) after 48 hours. This was significantly impaired in pregnant women (IFN-α; p<0.0001 and IFN-λ; p<0.001). Furthermore, in the presence of syncytin-1, PBMCs demonstrated marked reductions in IFN-α and IFN-λ, while enhanced release of IL-10 as well as IL-6 and IL-1β.<h4>Conclusions</h4>Our data indicates that a placental derived protein, syncytin-1 may be responsible for the heightened vulnerability of pregnant women to influenza. |
url |
https://doi.org/10.1371/journal.pone.0118629 |
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