Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection.

Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection.

The general methods to detect the RNA of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) in clinical diagnostic testing involve reverse transcriptases and thermostable DNA polymerases. In this study, we compared the detection of SARS-CoV-2 by a one-step real-time RT-PCR method using a h...

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Bibliographic Details
Main Authors: Yukiko Nakura, Heng Ning Wu, Yuya Okamoto, Muneyuki Takeuchi, Koichiro Suzuki, Yoshitaka Tamura, Yuichiro Oba, Fumiko Nishiumi, Nobuaki Hatori, Shinsuke Fujiwara, Kiyoshi Yasukawa, Shinobu Ida, Itaru Yanagihara
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0252789

Internet

https://doi.org/10.1371/journal.pone.0252789

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