Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen

Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen

Summary: This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurement...

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Bibliographic Details
Main Authors: Paul Dowling, Stephen Gargan, Margit Zweyer, Michael Henry, Paula Meleady, Dieter Swandulla, Kay Ohlendieck
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
High Throughput Screening
Model Organisms
Protein Biochemistry
Proteomics
Mass Spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301830

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http://www.sciencedirect.com/science/article/pii/S2666166720301830

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