Comparison of efficiency of various DNA extraction methods from cysts of Giardia intestinalis measured by PCR and TaqMan real time PCR

The aim of the presented study was to work out an effective method of extraction of DNA from Giardia intestinalis cysts as well as a sensitive and specific method for detection of DNA of this protozoan using a polymerase chain reaction (PCR). Twelve protocols for DNA extraction have been compared. P...

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Bibliographic Details
Main Authors: Adamska M., Leońska-Duniec A., Maciejewska A., Sawczuk M., Skotarczak B.
Format: Article
Language:English
Published: EDP Sciences 2010-12-01
Series:Parasite
Subjects:
PCR
Online Access:http://dx.doi.org/10.1051/parasite/2010174299