Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos.
The AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are un...
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doaj-2768985e5c834e6e97974fc4d1fb8b2b2020-11-24T20:51:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e10101510.1371/journal.pone.0101015Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos.Ryan D WaltersDavid T McSwiggenJames A GoodrichJennifer F KugelThe AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are unable to distinguish between the role of, for example, cJun/cJun homodimers versus cJun/cFos heterodimers. Here we used SELEX (systematic evolution of ligands by exponential enrichment) to identify and characterize DNA aptamers that are >100-fold more specific for binding cJun/cJun compared to cJun/cFos, setting the foundation to investigate the biological functions of different AP-1 dimer compositions.http://europepmc.org/articles/PMC4072727?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ryan D Walters David T McSwiggen James A Goodrich Jennifer F Kugel |
spellingShingle |
Ryan D Walters David T McSwiggen James A Goodrich Jennifer F Kugel Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos. PLoS ONE |
author_facet |
Ryan D Walters David T McSwiggen James A Goodrich Jennifer F Kugel |
author_sort |
Ryan D Walters |
title |
Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos. |
title_short |
Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos. |
title_full |
Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos. |
title_fullStr |
Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos. |
title_full_unstemmed |
Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos. |
title_sort |
selection and characterization of a dna aptamer that can discriminate between cjun/cjun and cjun/cfos. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
The AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are unable to distinguish between the role of, for example, cJun/cJun homodimers versus cJun/cFos heterodimers. Here we used SELEX (systematic evolution of ligands by exponential enrichment) to identify and characterize DNA aptamers that are >100-fold more specific for binding cJun/cJun compared to cJun/cFos, setting the foundation to investigate the biological functions of different AP-1 dimer compositions. |
url |
http://europepmc.org/articles/PMC4072727?pdf=render |
work_keys_str_mv |
AT ryandwalters selectionandcharacterizationofadnaaptamerthatcandiscriminatebetweencjuncjunandcjuncfos AT davidtmcswiggen selectionandcharacterizationofadnaaptamerthatcandiscriminatebetweencjuncjunandcjuncfos AT jamesagoodrich selectionandcharacterizationofadnaaptamerthatcandiscriminatebetweencjuncjunandcjuncfos AT jenniferfkugel selectionandcharacterizationofadnaaptamerthatcandiscriminatebetweencjuncjunandcjuncfos |
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1716801254484606976 |