Diagnosis of Brazilian vesiculoviruses by reverse transcription-polymerase chain reaction

We describe a reverse transcription-polymerase chain reaction (RT-PCR) and a nested-PCR for diagnosis of Piry, Carajás, Cocal, and Alagoas vesiculoviruses from Brazil. The RNA extracts of viral and clinical samples were submitted to a RT-PCR using Vesiculovirus G primers that amplify part of the gly...

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Bibliographic Details
Main Authors: Daniela Wey Bonutti, Luiz Tadeu Moraes Figueiredo
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2005-04-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000200014