Detection of spacer precursors formed in vivo during primed CRISPR adaptation

Primed adaptation in the CRISPR-Cas system helps recognition of previously encountered sequence elements and promotes the formation of new memories. Here the authors characterized spacer precursors of type I-E and type I-F CRISPR-Cas system using in vivo models.

Bibliographic Details
Main Authors:	Anna A. Shiriaeva, Ekaterina Savitskaya, Kirill A. Datsenko, Irina O. Vvedenskaya, Iana Fedorova, Natalia Morozova, Anastasia Metlitskaya, Anton Sabantsev, Bryce E. Nickels, Konstantin Severinov, Ekaterina Semenova
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-10-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-12417-w

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spelling	doaj-2843ec11149841ccb9221461a5c003302021-05-11T12:34:00ZengNature Publishing GroupNature Communications2041-17232019-10-011011910.1038/s41467-019-12417-wDetection of spacer precursors formed in vivo during primed CRISPR adaptationAnna A. Shiriaeva0Ekaterina Savitskaya1Kirill A. Datsenko2Irina O. Vvedenskaya3Iana Fedorova4Natalia Morozova5Anastasia Metlitskaya6Anton Sabantsev7Bryce E. Nickels8Konstantin Severinov9Ekaterina Semenova10Center of Life Sciences, Skolkovo Institute of Science and TechnologyCenter of Life Sciences, Skolkovo Institute of Science and TechnologyDepartment of Molecular Biology and Biochemistry, Waksman Institute, Rutgers UniversityDepartment of Genetics, Waksman Institute, Rutgers UniversityCenter of Life Sciences, Skolkovo Institute of Science and TechnologyCenter of Life Sciences, Skolkovo Institute of Science and TechnologyInstitute of Molecular Genetics, Russian Academy of SciencesPeter the Great St. Petersburg Polytechnic UniversityDepartment of Genetics, Waksman Institute, Rutgers UniversityCenter of Life Sciences, Skolkovo Institute of Science and TechnologyDepartment of Molecular Biology and Biochemistry, Waksman Institute, Rutgers UniversityPrimed adaptation in the CRISPR-Cas system helps recognition of previously encountered sequence elements and promotes the formation of new memories. Here the authors characterized spacer precursors of type I-E and type I-F CRISPR-Cas system using in vivo models.https://doi.org/10.1038/s41467-019-12417-w
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Anna A. Shiriaeva Ekaterina Savitskaya Kirill A. Datsenko Irina O. Vvedenskaya Iana Fedorova Natalia Morozova Anastasia Metlitskaya Anton Sabantsev Bryce E. Nickels Konstantin Severinov Ekaterina Semenova
spellingShingle	Anna A. Shiriaeva Ekaterina Savitskaya Kirill A. Datsenko Irina O. Vvedenskaya Iana Fedorova Natalia Morozova Anastasia Metlitskaya Anton Sabantsev Bryce E. Nickels Konstantin Severinov Ekaterina Semenova Detection of spacer precursors formed in vivo during primed CRISPR adaptation Nature Communications
author_facet	Anna A. Shiriaeva Ekaterina Savitskaya Kirill A. Datsenko Irina O. Vvedenskaya Iana Fedorova Natalia Morozova Anastasia Metlitskaya Anton Sabantsev Bryce E. Nickels Konstantin Severinov Ekaterina Semenova
author_sort	Anna A. Shiriaeva
title	Detection of spacer precursors formed in vivo during primed CRISPR adaptation
title_short	Detection of spacer precursors formed in vivo during primed CRISPR adaptation
title_full	Detection of spacer precursors formed in vivo during primed CRISPR adaptation
title_fullStr	Detection of spacer precursors formed in vivo during primed CRISPR adaptation
title_full_unstemmed	Detection of spacer precursors formed in vivo during primed CRISPR adaptation
title_sort	detection of spacer precursors formed in vivo during primed crispr adaptation
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-10-01
description	Primed adaptation in the CRISPR-Cas system helps recognition of previously encountered sequence elements and promotes the formation of new memories. Here the authors characterized spacer precursors of type I-E and type I-F CRISPR-Cas system using in vivo models.
url	https://doi.org/10.1038/s41467-019-12417-w
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Detection of spacer precursors formed in vivo during primed CRISPR adaptation

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