Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes

Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes

Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are rev...

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Bibliographic Details
Main Authors: Hannah L Klein, Kenny K.H. Ang, Michelle R. Arkin, Emily C. Beckwitt, Yi-Hsuan Chang, Jun Fan, Youngho Kwon, Michael J. Morten, Sucheta Mukherjee, Oliver J. Pambos, Hafez el Sayyed, Elizabeth S. Thrall, João P. Vieira-da-Rocha, Quan Wang, Shuang Wang, Hsin-Yi Yeh, Julie S. Biteen, Peter Chi, Wolf-Dietrich Heyer, Achillefs N. Kapanidis, Joseph J. Loparo, Terence R. Strick, Patrick Sung, Bennett Van Houten, Hengyao Niu, Eli Rothenberg
Format: Article
Language:English
Published: Shared Science Publishers OG 2019-01-01
Series:Microbial Cell
Subjects:
chromatin dynamics
chromosome rearrangements
crossovers
DNA breaks
DNA helicases
DNA repair centers
DNA repair synthesis
DNA resection
double strand break repair
DSBs
endonuclease protection assay
genome instability
gross chromosome rearrangements
fluorescent proteins
FRET
homologous recombination
mismatch repair
nonhomologous end joining
nucleotide excision repair
PALM
photoactivated fluorescent proteins
recombinase filament assembly
single-molecule
single-particle tracking
super resolution
structure-selective endonucleases
synthesis-dependent strand annealing
transcription coupled repair
Online Access:http://microbialcell.com/researcharticles/2019b-klein-microbial-cell/

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http://microbialcell.com/researcharticles/2019b-klein-microbial-cell/

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