Development of novel parameter for monitoring of malignant melanoma progression
Objective: Increasing HIFs in malignant melanoma, the highly aggressive skin tumour, results in the stimulation of invasiveness. Increased HIF-1α fallouts in inhibition of the activity of some mitochondrial enzymes and leads to preference of cytosol energetic metabolism. Increase of aerobic glycolys...
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doaj-284d891bfc064e14bf25a62595b24f0e2020-12-21T04:45:42ZengElsevierPractical Laboratory Medicine2352-55172020-11-0122e00182Development of novel parameter for monitoring of malignant melanoma progressionIvana Špaková0Miroslava Rabajdová1Katarína Dubayová2Vladimíra Nagyová3Martina Bago Pilátová4Mária Mareková5Department of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Medical Faculty, Slovakia; Corresponding author. Department of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University, Medical Faculty, Trieda SNP 1; 040 11, Košice, Slovakia.Department of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Medical Faculty, SlovakiaDepartment of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Medical Faculty, SlovakiaDepartment of Dermatovenerology, Pavol Jozef Šafárik University in Košice, Medical Faculty, SlovakiaDepartment of Pharmacology, Pavol Jozef Šafárik University in Košice, Medical Faculty, SlovakiaDepartment of Medical and Clinical Biochemistry, Pavol Jozef Šafárik University in Košice, Medical Faculty, SlovakiaObjective: Increasing HIFs in malignant melanoma, the highly aggressive skin tumour, results in the stimulation of invasiveness. Increased HIF-1α fallouts in inhibition of the activity of some mitochondrial enzymes and leads to preference of cytosol energetic metabolism. Increase of aerobic glycolysis is reflected in an increase of free NADH (Warburg effect) and develops the malignant melanoma.Our goal was to find a link between hypoxia, or hypoxia mimicking factors and the stage of malignant melanoma. Furthermore, we focused on the finding of the experimental parameter which could monitor melanoma patients. Patients and methods: We targeted HIF-1α gene expression and VDR rs2107301 gene polymorphism by PCR analysis. We detected the level of NADH in blood plasma by fluorescence spectroscopy (excitation and emission spectra). Results: Analysis of the obtained data from patient samples has shown an increase in HIF-1α which correlates with the disease stage. Investigation VDR rs2107301 polymorphism of patient samples does not show any significant changes in single nucleotide polymorphism, and the low vitamin D level in blood is not a result of VDR mutation in mitochondria. NADH levels vary under hypoxic and pseudohypoxic conditions and refer to the cancer stage. Conclusions: The apparent mismatch between HIF-1α expression and NADH fluorescence has become the basis for the design of an algorithm for monitoring malignant melanoma based on the sensing of NADH fluorescence and the determination of HIF-1α.http://www.sciencedirect.com/science/article/pii/S2352551720301451Malignant melanomaHIF-1αNADHVDRVitamin D |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ivana Špaková Miroslava Rabajdová Katarína Dubayová Vladimíra Nagyová Martina Bago Pilátová Mária Mareková |
spellingShingle |
Ivana Špaková Miroslava Rabajdová Katarína Dubayová Vladimíra Nagyová Martina Bago Pilátová Mária Mareková Development of novel parameter for monitoring of malignant melanoma progression Practical Laboratory Medicine Malignant melanoma HIF-1α NADH VDR Vitamin D |
author_facet |
Ivana Špaková Miroslava Rabajdová Katarína Dubayová Vladimíra Nagyová Martina Bago Pilátová Mária Mareková |
author_sort |
Ivana Špaková |
title |
Development of novel parameter for monitoring of malignant melanoma progression |
title_short |
Development of novel parameter for monitoring of malignant melanoma progression |
title_full |
Development of novel parameter for monitoring of malignant melanoma progression |
title_fullStr |
Development of novel parameter for monitoring of malignant melanoma progression |
title_full_unstemmed |
Development of novel parameter for monitoring of malignant melanoma progression |
title_sort |
development of novel parameter for monitoring of malignant melanoma progression |
publisher |
Elsevier |
series |
Practical Laboratory Medicine |
issn |
2352-5517 |
publishDate |
2020-11-01 |
description |
Objective: Increasing HIFs in malignant melanoma, the highly aggressive skin tumour, results in the stimulation of invasiveness. Increased HIF-1α fallouts in inhibition of the activity of some mitochondrial enzymes and leads to preference of cytosol energetic metabolism. Increase of aerobic glycolysis is reflected in an increase of free NADH (Warburg effect) and develops the malignant melanoma.Our goal was to find a link between hypoxia, or hypoxia mimicking factors and the stage of malignant melanoma. Furthermore, we focused on the finding of the experimental parameter which could monitor melanoma patients. Patients and methods: We targeted HIF-1α gene expression and VDR rs2107301 gene polymorphism by PCR analysis. We detected the level of NADH in blood plasma by fluorescence spectroscopy (excitation and emission spectra). Results: Analysis of the obtained data from patient samples has shown an increase in HIF-1α which correlates with the disease stage. Investigation VDR rs2107301 polymorphism of patient samples does not show any significant changes in single nucleotide polymorphism, and the low vitamin D level in blood is not a result of VDR mutation in mitochondria. NADH levels vary under hypoxic and pseudohypoxic conditions and refer to the cancer stage. Conclusions: The apparent mismatch between HIF-1α expression and NADH fluorescence has become the basis for the design of an algorithm for monitoring malignant melanoma based on the sensing of NADH fluorescence and the determination of HIF-1α. |
topic |
Malignant melanoma HIF-1α NADH VDR Vitamin D |
url |
http://www.sciencedirect.com/science/article/pii/S2352551720301451 |
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1724375669425569792 |