Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish

Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has been proven to be an efficient and precise genome editing technology in various organisms. However, the gene editing efficiencies of Cas9 proteins with a nuclear localization signal (NLS) fused to different termin...

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Bibliographic Details
Main Authors: Peinan Hu, Xueying Zhao, Qinghua Zhang, Weiming Li, Yao Zu
Format: Article
Language:English
Published: Oxford University Press 2018-03-01
Series:G3: Genes, Genomes, Genetics
Subjects:
CRISPR/Cas9
Cas9 protein
knockout
efficiency
NLS
zebrafish
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.117.300359

Internet

http://g3journal.org/lookup/doi/10.1534/g3.117.300359

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