Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris

Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris

Pichia pastoris (Komagataella sp.) is broadly used for the production of secreted recombinant proteins. Due to the high rate of protein production, incorrectly folded proteins may accumulate in the endoplasmic reticulum (ER). To restore their proper folding, the cell triggers the unfolded protein re...

Full description

Bibliographic Details
Main Authors: Hana Raschmanová, Iwo Zamora, Martina Borčinová, Patrick Meier, Astrid Weninger, Dominik Mächler, Anton Glieder, Karel Melzoch, Zdeněk Knejzlík, Karin Kovar
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-02-01
Series:Frontiers in Microbiology
Subjects:
unfolded protein response (UPR)
stress response
Pichia pastoris
super folder green fluorescent protein (sfGFP)
flow cytometry
single-cell
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00335/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.00335/full

Similar Items