Chloroplast sequence variation and the efficacy of peptide nucleic acids for blocking host amplification in plant microbiome studies

Chloroplast sequence variation and the efficacy of peptide nucleic acids for blocking host amplification in plant microbiome studies

Abstract Background The ability to efficiently characterize microbial communities from host individuals can be limited by co-amplification of host organellar sequences (mitochondrial and/or plastid), which share a common ancestor and thus sequence similarity with extant bacterial lineages. One promi...

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Bibliographic Details
Main Authors: Connor R. Fitzpatrick, Patricia Lu-Irving, Julia Copeland, David S. Guttman, Pauline W. Wang, David A. Baltrus, Katrina M. Dlugosch, Marc T. J. Johnson
Format: Article
Language:English
Published: BMC 2018-08-01
Series:Microbiome
Subjects:
Plastid variation
PNA clamp
Host contamination
16S amplicon sequencing
Asteraceae
Online Access:http://link.springer.com/article/10.1186/s40168-018-0534-0

Internet

http://link.springer.com/article/10.1186/s40168-018-0534-0

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