McFarland nephelometer as a simple method to estimate the sensitivity of the polymerase chain reaction using Mycobacterium tuberculosis as a research tool

Polymerase chain reaction (PCR) has been widely investigated for the diagnosis of tuberculosis. However, before this technique is applied on clinical samples, it needs to be well standardized. We describe the use of McFarland nephelometer, a very simple approach to determine microorganism concentrat...

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Bibliographic Details
Main Authors: Bollela V.R., Sato D.N., Fonseca B.A.L.
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 1999-01-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
PCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900003