CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies

CRISPRi enables isoform-specific loss-of-function screens and identification of gastric cancer-specific isoform dependencies

Abstract Introduction Genes contain multiple promoters that can drive the expression of various transcript isoforms. Although transcript isoforms from the same gene could have diverse and non-overlapping functions, current loss-of-function methodologies are not able to differentiate between isoform-...

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Main Authors: Rebecca Davies, Ling Liu, Sheng Taotao, Natasha Tuano, Richa Chaturvedi, Kie Kyon Huang, Catherine Itman, Amit Mandoli, Aditi Qamra, Changyuan Hu, David Powell, Roger J. Daly, Patrick Tan, Joseph Rosenbluh
Format: Article
Language:English
Published: BMC 2021-01-01
Series:Genome Biology
Online Access:https://doi.org/10.1186/s13059-021-02266-6

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https://doi.org/10.1186/s13059-021-02266-6

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