Characterization of AAV-Specific Affinity Ligands: Consequences for Vector Purification and Development Strategies

Characterization of AAV-Specific Affinity Ligands: Consequences for Vector Purification and Development Strategies

Affinity-based purification of adeno-associated virus (AAV) vectors has replaced density-based methods for vectors used in clinical settings. This method utilizes camelid single-domain antibodies recognizing AAV capsids. These include AVB Sepharose (AVB) and POROS CaptureSelect affinity ligand for A...

Full description

Bibliographic Details
Main Authors: Mario Mietzsch, J. Kennon Smith, Jennifer C. Yu, Vibhu Banala, Shanan N. Emmanuel, Ariana Jose, Paul Chipman, Nilakshee Bhattacharya, Robert McKenna, Mavis Agbandje-McKenna
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
adeno-associated virus (AAV)
vector purification
affinity chromatography
nanobodies
cryo-electron microscopy and image reconstruction
gene therapy
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050120302059

Internet

http://www.sciencedirect.com/science/article/pii/S2329050120302059

Similar Items