Characterization and applications of nanobodies against Pseudomonas aeruginosa Exotoxin A selected from single alpaca B cells

Characterization and applications of nanobodies against Pseudomonas aeruginosa Exotoxin A selected from single alpaca B cells

We have established a highly efficient 96-well format based strategy to characterize nanobody (Nb) from an expressed alpaca Nb repertoire by combining heavy-chain antibody variable region gene cloning with Nb expression and reactivity profiling at the single cell level. Individual alpaca B lymphocyt...

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Bibliographic Details
Main Authors: Jingtao Lin, Yi Gu, Yanru Xu, Jianli Yu, Jinsong Tang, Lili Wu, Zhengwei Zhou, Cailing Chen, Minjuan Liu, Xuan Chun, Hongling Liu, Rui Nian, Haipeng Song, Jing Zhang
Format: Article
Language:English
Published: Taylor & Francis Group 2020-01-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
nanobody
facs
single cell
nested pcr
rapid cloning
Online Access:http://dx.doi.org/10.1080/13102818.2020.1817782

Internet

http://dx.doi.org/10.1080/13102818.2020.1817782

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