Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

Abstract Heterologous protein production is widely used in industrial biotechnology. However, using non-native production hosts can lead to enzymes with altered post-translational modifications, such as glycosylation. We have investigated how production in a non-native host affects the physicochemic...

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Bibliographic Details
Main Authors: Cyrielle Bonzom, Silvia Hüttner, Ekaterina Mirgorodskaya, Sun-Li Chong, Stefan Uthoff, Alexander Steinbüchel, Raymond M. D. Verhaert, Lisbeth Olsson
Format: Article
Language:English
Published: SpringerOpen 2019-08-01
Series:AMB Express
Subjects:
Mass spectrometry (MS)
Enzyme activity
Enzyme stability
Heterologous production
Escherichia coli
Pichia pastoris
Online Access:http://link.springer.com/article/10.1186/s13568-019-0852-z

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http://link.springer.com/article/10.1186/s13568-019-0852-z

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