CRISPR/Cas12a-mediated labeling of MET receptor enables quantitative single-molecule imaging of endogenous protein organization and dynamics

CRISPR/Cas12a-mediated labeling of MET receptor enables quantitative single-molecule imaging of endogenous protein organization and dynamics

Summary: Single-molecule localization microscopy (SMLM) reports on protein organization in cells with near-molecular resolution and in combination with stoichiometric labeling enables protein counting. Fluorescent proteins allow stoichiometric labeling of cellular proteins; however, most methods eit...

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Bibliographic Details
Main Authors: Tim N. Baldering, Christos Karathanasis, Marie-Lena I.E. Harwardt, Petra Freund, Matthias Meurer, Johanna V. Rahm, Michael Knop, Marina S. Dietz, Mike Heilemann
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:iScience
Subjects:
Biochemistry
Molecular Biology
Biophysics
Biological Sciences Research Methodologies
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004220310920

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http://www.sciencedirect.com/science/article/pii/S2589004220310920

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