Cloning, expression and characterization of the endoglucanase gene from Bacillus subtilis UMC7 isolated from the gut of the indigenous termite Macrotermes malaccensis in Escherichia coli

Cloning, expression and characterization of the endoglucanase gene from Bacillus subtilis UMC7 isolated from the gut of the indigenous termite Macrotermes malaccensis in Escherichia coli

Background: Bacillus subtilis UMC7 isolated from the gut of termite Macrotermes malaccensis has the ability to secrete a significant amount of extracellular endoglucanase, with an enzyme activity of 0.12 ± 0.01 μmol/min/mL. However, for economically viable industrial applications, the enzyme needs t...

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Bibliographic Details
Main Authors: Kelvin Swee Chuan Wei, Teow Chong Teoh, Philip Koshy, Ismail Salmah, Arifin Zainudin
Format: Article
Language:English
Published: Elsevier 2015-03-01
Series:Electronic Journal of Biotechnology
Subjects:
β-1,4-Endoglucanase
Carboxyl-methylcellulose
Extracellular enzyme activity
Metal ions effect
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345814001523

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http://www.sciencedirect.com/science/article/pii/S0717345814001523

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