Methodological variations in the isolation of genomic DNA from Streptococcus bacteria

In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was...

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Bibliographic Details
Main Authors: Mônica Moreira, Juliana Noschang, Ivana Froede Neiva, Yanê Carvalho, llma Hiroko Higuti, Vânia Aparecida Vicente
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2010-08-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013