Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Instituto de Tecnologia do Paraná (Tecpar)
2010-08-01
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Series: | Brazilian Archives of Biology and Technology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013 |