Chimeric Phi29 DNA polymerase with helix–hairpin–helix motifs shows enhanced salt tolerance and replication performance

Summary Phi29 DNA polymerase (Phi29 Pol) has been successfully applied in DNA nanoball‐based sequencing, real‐time DNA sequencing from single polymerase molecules and nanopore sequencing employing the sequencing by synthesis (SBS) method. Among these, polymerase‐assisted nanopore sequencing technolo...

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Bibliographic Details
Main Authors: Yaping Gao, Yun He, Liyi Chen, Xing Liu, Igor Ivanov, Xuerui Yang, Hui Tian
Format: Article
Language:English
Published: Wiley 2021-07-01
Series:Microbial Biotechnology
Online Access:https://doi.org/10.1111/1751-7915.13830