Engineered Streptomyces lividans Strains for Optimal Identification and Expression of Cryptic Biosynthetic Gene Clusters

Engineered Streptomyces lividans Strains for Optimal Identification and Expression of Cryptic Biosynthetic Gene Clusters

Streptomyces lividans is a suitable host for the heterologous expression of biosynthetic gene clusters (BGCs) from actinomycetes to discover “cryptic” secondary metabolites. To improve the heterologous expression of BGCs, herein we optimized S. lividans strain SBT5 via the stepwise integration of th...

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Bibliographic Details
Main Authors: Qinying Peng, Guixi Gao, Jin Lü, Qingshan Long, Xuefei Chen, Fei Zhang, Min Xu, Kai Liu, Yemin Wang, Zixin Deng, Zhiyong Li, Meifeng Tao
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-12-01
Series:Frontiers in Microbiology
Subjects:
optimal hosts
global regulatory genes
heterologous expression
biosynthetic gene clusters (BGCs)
secondary metabolites
library expression and function-directed screening system (LEXAS)
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.03042/full

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https://www.frontiersin.org/article/10.3389/fmicb.2018.03042/full

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