Harnessing the Endogenous 2μ Plasmid of Saccharomyces cerevisiae for Pathway Construction

pRS episomal plasmids are widely used in Saccharomyces cerevisiae, owing to their easy genetic manipulations and high plasmid copy numbers (PCNs). Nevertheless, their broader application is hampered by the instability of the pRS plasmids. In this study, we designed an episomal plasmid based on the e...

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Bibliographic Details
Main Authors: Jing Yang, Yujuan Tian, Huayi Liu, Yeyi Kan, Yi Zhou, Ying Wang, Yunzi Luo
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.679665/full