Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS

Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS

Abstract Background Recombinant adeno-associated virus (rAAV) is widely used in the neuroscience field to manipulate gene expression in the nervous system. However, a limitation to the use of rAAV vectors is the time and expense needed to produce them. To overcome this limitation, we evaluated wheth...

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Bibliographic Details
Main Authors: Marshall S. Goodwin, Cara L. Croft, Hunter S. Futch, Daniel Ryu, Carolina Ceballos-Diaz, Xuefei Liu, Giavanna Paterno, Catalina Mejia, Doris Deng, Kimberly Menezes, Laura Londono, Kefren Arjona, Mary Parianos, Van Truong, Eva Rostonics, Amanda Hernandez, Sanford L. Boye, Shannon E. Boye, Yona Levites, Pedro E. Cruz, Todd E. Golde
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Molecular Neurodegeneration
Subjects:
Adeno-associated virus
Capsid pseudo-type
AAV production
Gene delivery
Central nervous system
Online Access:http://link.springer.com/article/10.1186/s13024-020-00361-z

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http://link.springer.com/article/10.1186/s13024-020-00361-z

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