Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching

Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching

Super-resolution microscopy with spontaneously blinking dyes is dependent on pH and polarity of the medium. Here the authors introduce a photoactivatable fluxional fluorophore for live cell imaging that allows control over the fraction of spontaneously blinking molecules independently of medium prop...

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Bibliographic Details
Main Authors: Elias A. Halabi, Dorothea Pinotsi, Pablo Rivera-Fuentes
Format: Article
Language:English
Published: Nature Publishing Group 2019-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-09217-7
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spelling doaj-2a41a7ef91e445dda79340cd22f6a3512021-01-31T15:17:47ZengNature Publishing GroupNature Communications2041-17232019-03-0110111010.1038/s41467-019-09217-7Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleachingElias A. Halabi0Dorothea Pinotsi1Pablo Rivera-Fuentes2Laboratory of Organic Chemistry, ETH ZurichScientific Center for Optical and Electron Microscopy, ETH ZurichLaboratory of Organic Chemistry, ETH ZurichSuper-resolution microscopy with spontaneously blinking dyes is dependent on pH and polarity of the medium. Here the authors introduce a photoactivatable fluxional fluorophore for live cell imaging that allows control over the fraction of spontaneously blinking molecules independently of medium properties.https://doi.org/10.1038/s41467-019-09217-7
collection DOAJ
language English
format Article
sources DOAJ
author Elias A. Halabi
Dorothea Pinotsi
Pablo Rivera-Fuentes
spellingShingle Elias A. Halabi
Dorothea Pinotsi
Pablo Rivera-Fuentes
Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
Nature Communications
author_facet Elias A. Halabi
Dorothea Pinotsi
Pablo Rivera-Fuentes
author_sort Elias A. Halabi
title Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
title_short Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
title_full Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
title_fullStr Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
title_full_unstemmed Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
title_sort photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-03-01
description Super-resolution microscopy with spontaneously blinking dyes is dependent on pH and polarity of the medium. Here the authors introduce a photoactivatable fluxional fluorophore for live cell imaging that allows control over the fraction of spontaneously blinking molecules independently of medium properties.
url https://doi.org/10.1038/s41467-019-09217-7
work_keys_str_mv AT eliasahalabi photoregulatedfluxionalfluorophoresforlivecellsuperresolutionmicroscopywithnoapparentphotobleaching
AT dorotheapinotsi photoregulatedfluxionalfluorophoresforlivecellsuperresolutionmicroscopywithnoapparentphotobleaching
AT pabloriverafuentes photoregulatedfluxionalfluorophoresforlivecellsuperresolutionmicroscopywithnoapparentphotobleaching
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