Suppression of <i>SHROOM1</i> Improves In Vitro and In Vivo Gene Integration by Promoting Homology-Directed Repair

Suppression of <i>SHROOM1</i> Improves In Vitro and In Vivo Gene Integration by Promoting Homology-Directed Repair

Homologous recombination (HR) is often used to achieve targeted gene integration because of its higher precision and operability compared with microhomology-mediated end-joining (MMEJ) or non-homologous end-joining (NHEJ). It appears to be inefficient for gene integration in animal cells and embryos...

Full description

Bibliographic Details
Main Authors: Zhihua Zhao, Hanshuo Zhang, Tuanlin Xiong, Junyi Wang, Di Yang, Dan Zhu, Juan Li, Ye Yang, Changhong Sun, Yuting Zhao, Jianzhong Jeff Xi
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:International Journal of Molecular Sciences
Subjects:
homologous recombination
<i>SHROOM1</i>
knock-in
gene editing
CRISPR/Cas9
Online Access:https://www.mdpi.com/1422-0067/21/16/5821

Internet

https://www.mdpi.com/1422-0067/21/16/5821

Similar Items