A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria

A Host-Specific Blocking Primer Combined with Optimal DNA Extraction Improves the Detection Capability of a Metabarcoding Protocol for Canine Vector-Borne Bacteria

Bacterial canine vector-borne diseases are responsible for some of the most life-threatening conditions of dogs in the tropics and are typically poorly researched with some presenting a zoonotic risk to cohabiting people. Next-generation sequencing based methodologies have been demonstrated to accur...

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Bibliographic Details
Main Authors: Lucas G. Huggins, Anson V. Koehler, Bettina Schunack, Tawin Inpankaew, Rebecca J. Traub
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Pathogens
Subjects:
canine vector-borne disease
blocking primers
blood DNA extraction
next-generation sequencing
kit contaminant bacteria
Online Access:https://www.mdpi.com/2076-0817/9/4/258

Internet

https://www.mdpi.com/2076-0817/9/4/258

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