Enzymatic and Acid Hydrolysis of Sago Starch for Preparation of Ethanol Production

A series of studies on the hydrolysis of Sago starch for ethanol fermentation had been conducted. Hydrolysis of sago starch was carried out using sulfuric acid 2.5% and amylase(s) enzymes. The concentrations of sago starch used in this experiment were  5, 10, 15, 20, and 30% (w/v). The highest hydr...

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Bibliographic Details
Main Authors: ROFIQ SUNARYANTO, BERTI HARIASIH HANDAYANI, RATU SAFITRI
Format: Article
Language:English
Published: Indonesian Society for Microbiology 2013-07-01
Series:Microbiology Indonesia
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/213
Description
Summary:A series of studies on the hydrolysis of Sago starch for ethanol fermentation had been conducted. Hydrolysis of sago starch was carried out using sulfuric acid 2.5% and amylase(s) enzymes. The concentrations of sago starch used in this experiment were  5, 10, 15, 20, and 30% (w/v). The highest hydrolyzate  containing reducing sugar was used as substrate for ethanol fermentation by Saccharomyces cerevisiae FNCC 3012. The results indicated  that hydrolysis using 2.5% sulfuric acid for 120 min at 121 °C produced 6.6%  (w/v)  reducing sugar and hydrolysis using α-amylase and Dextrozyme DX produced more reducing sugar, 7% (w/v) and 17.1% (w/v), respectively. The fermentation of hydrolyzed sago starch by S. cerevisiae FNCC 3012 produced ethanol 7.98% (v/v).
ISSN:1978-3477
2087-8575