Use of the GRP1 PH domain as a tool to measure the relative levels of PtdIns(3,4,5)P3 through a protein-lipid overlay approach

Use of the GRP1 PH domain as a tool to measure the relative levels of PtdIns(3,4,5)P3 through a protein-lipid overlay approach

We describe a novel approach to the relative quantification of phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5)P3] and its application to measure, in neutrophils, the activation of phosphoinositide 3-kinase (PI3K). This protein-lipid overlay-based assay allowed us to confirm and extend the...

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Bibliographic Details
Main Authors: Hervé Guillou, Charlotte Lécureuil, Karen E. Anderson, Sabine Suire, G. John Ferguson, Chris D. Ellson, Alexander Gray, Nullin Divecha, Phillip T. Hawkins, Len R. Stephens
Format: Article
Language:English
Published: Elsevier 2007-03-01
Series:Journal of Lipid Research
Subjects:
phosphatidylinositol (3,4,5)-trisphosphate
neomycin
phosphoinositide 3-kinase
general receptor for phosphoinositides-1
protein-lipid overlay
neutrophil
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520432481

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http://www.sciencedirect.com/science/article/pii/S0022227520432481

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