Monitoring of tisagenlecleucel transgene DNA using a quantitative polymerase chain reaction assay

Monitoring of tisagenlecleucel transgene DNA using a quantitative polymerase chain reaction assay

Chimeric antigen receptor (CAR)-T cell therapies reprogram T cells to engage and eliminate cancer cells. Patients’ T cells are transduced in vitro using lentiviral or retroviral vectors containing a CAR transgene. Following infusion, CAR-T cells expand in vivo and may persist in the peripheral blood...

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Bibliographic Details
Main Authors: Lisa Davis, Nathan Riccitelli, Nancy Valencia, Irene L. Ch’en, Shabnam Tangri, Jennifer L. Brogdon, Creton Kalfoglou, Karen Thudium Mueller, Reinhold Pollner
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
CAR-T cell therapy
CAR transgene
qPCR
cellular kinetics
expansion
persistence
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050120302503

Internet

http://www.sciencedirect.com/science/article/pii/S2329050120302503

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