Development and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples
Abstract A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
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Series: | Brazilian Journal of Microbiology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300584&lng=en&tlng=en |