Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during Ripening
The aim of the present study was to assess the influence of ripening container’s material on the bacterial diversity of Feta cheese PDO (Protected Designation of Origin). The microbiota of fresh and mature cheese produced in plastic and stainless steel container was monitored by microbial enumeratio...
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doaj-2bc1a6af83bb45de87a2a78184394a2a2020-11-25T02:33:37ZengMDPI AGFermentation2311-56372020-01-01611210.3390/fermentation6010012fermentation6010012Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during RipeningEvgenia D. Spyrelli0Anastasios Stamatiou1Chrysoula C. Tassou2George-John E. Nychas3Agapi I. Doulgeraki4Department of Food Science and Human Nutrition, Laboratory of Microbiology and Biotechnology of Foods, Agricultural University of Athens (AUA), Iera Odos 75, 11855 Athens, GreeceDepartment of Food Science and Human Nutrition, Laboratory of Microbiology and Biotechnology of Foods, Agricultural University of Athens (AUA), Iera Odos 75, 11855 Athens, GreeceInstitute of Technology of Agricultural Products, Hellenic Agricultural Organization—DEMETER, Sof. Venizelou 1, Lycovrissi, 14123 Attica, GreeceDepartment of Food Science and Human Nutrition, Laboratory of Microbiology and Biotechnology of Foods, Agricultural University of Athens (AUA), Iera Odos 75, 11855 Athens, GreeceInstitute of Technology of Agricultural Products, Hellenic Agricultural Organization—DEMETER, Sof. Venizelou 1, Lycovrissi, 14123 Attica, GreeceThe aim of the present study was to assess the influence of ripening container’s material on the bacterial diversity of Feta cheese PDO (Protected Designation of Origin). The microbiota of fresh and mature cheese produced in plastic and stainless steel container was monitored by microbial enumeration and 16s rRNA gene sequencing. According to the obtained results, lactic acid bacteria (LAB) was the dominant microbiota of fresh and mature cheese. Metagenomics data revealed that fresh cheese was dominated by <i>Lactococcus</i> followed by members of <i>Enterobacteriaceae</i> family and <i>Pseudomonas</i>. Similarly, <i>Lactococcus</i> was the most abundant genus detected in mature cheese (54 days and 120 days), regardless of the container’s material. In both fresh and mature cheese, species of <i>Pseudomonas</i>, <i>Streptococcus</i>, <i>Acinetobacter</i>, <i>Lactobacillus</i>, <i>Flavobacterium</i>, and <i>Carnobacterium</i> were detected. The abundance of <i>Enterobacteriaceae</i>, <i>Moraxellaceae</i> and <i>Pseudomonadaceae</i> in mature cheese ripened in stainless steel container seems to be numerically reduced after 120 days of storage compared to the cheese ripened in plastic container but not significant differences were observed (<i>p</i> > 0.05). In conclusion, metagenomic analysis suggests that ripening container’s material does not affect the microbial community responsible for the ripening of feta cheese PDO.https://www.mdpi.com/2311-5637/6/1/12feta cheese pdomicrobiotadiversitynext generation sequencing<i>lactococcus</i>cheese ripening |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Evgenia D. Spyrelli Anastasios Stamatiou Chrysoula C. Tassou George-John E. Nychas Agapi I. Doulgeraki |
spellingShingle |
Evgenia D. Spyrelli Anastasios Stamatiou Chrysoula C. Tassou George-John E. Nychas Agapi I. Doulgeraki Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during Ripening Fermentation feta cheese pdo microbiota diversity next generation sequencing <i>lactococcus</i> cheese ripening |
author_facet |
Evgenia D. Spyrelli Anastasios Stamatiou Chrysoula C. Tassou George-John E. Nychas Agapi I. Doulgeraki |
author_sort |
Evgenia D. Spyrelli |
title |
Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during Ripening |
title_short |
Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during Ripening |
title_full |
Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during Ripening |
title_fullStr |
Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during Ripening |
title_full_unstemmed |
Microbiological and Metagenomic Analysis to Assess the Effect of Container Material on the Microbiota of Feta Cheese during Ripening |
title_sort |
microbiological and metagenomic analysis to assess the effect of container material on the microbiota of feta cheese during ripening |
publisher |
MDPI AG |
series |
Fermentation |
issn |
2311-5637 |
publishDate |
2020-01-01 |
description |
The aim of the present study was to assess the influence of ripening container’s material on the bacterial diversity of Feta cheese PDO (Protected Designation of Origin). The microbiota of fresh and mature cheese produced in plastic and stainless steel container was monitored by microbial enumeration and 16s rRNA gene sequencing. According to the obtained results, lactic acid bacteria (LAB) was the dominant microbiota of fresh and mature cheese. Metagenomics data revealed that fresh cheese was dominated by <i>Lactococcus</i> followed by members of <i>Enterobacteriaceae</i> family and <i>Pseudomonas</i>. Similarly, <i>Lactococcus</i> was the most abundant genus detected in mature cheese (54 days and 120 days), regardless of the container’s material. In both fresh and mature cheese, species of <i>Pseudomonas</i>, <i>Streptococcus</i>, <i>Acinetobacter</i>, <i>Lactobacillus</i>, <i>Flavobacterium</i>, and <i>Carnobacterium</i> were detected. The abundance of <i>Enterobacteriaceae</i>, <i>Moraxellaceae</i> and <i>Pseudomonadaceae</i> in mature cheese ripened in stainless steel container seems to be numerically reduced after 120 days of storage compared to the cheese ripened in plastic container but not significant differences were observed (<i>p</i> > 0.05). In conclusion, metagenomic analysis suggests that ripening container’s material does not affect the microbial community responsible for the ripening of feta cheese PDO. |
topic |
feta cheese pdo microbiota diversity next generation sequencing <i>lactococcus</i> cheese ripening |
url |
https://www.mdpi.com/2311-5637/6/1/12 |
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