Creation of Tenecteplase-Producing CHO Cell Line Using Site-Specific Integrase from the Phage φC31

Creation of Tenecteplase-Producing CHO Cell Line Using Site-Specific Integrase from the Phage φC31

Objective: The aim of this study was to produce a stable CHO cell line expressing tenecteplase.Materials and Methods: In the first step, the tenecteplase coding sequence was clonedin a pDB2 vector containing attB recognition sites for the phage φC31 integrase. Then,using lipofection, the CHO cells w...

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Bibliographic Details
Main Authors: Khadijeh Karbalaie, Mohammad Reza Mofid, Mohammad Hossain Nasr Esfahani, Michele P. Calos, Kianoush Dormiani, Pharm, Fereshteh Karamali, Kamran Ghaedi, Mahboobeh Forouzanfar, Yahya Khazaie, Pharm
Format: Article
Language:English
Published: Royan Institute (ACECR), Tehran 2010-01-01
Series:Cell Journal
Subjects:
Tenecteplase
Integrase
Pubmed
CHO
Online Access:http://celljournal.org/library/upload/article/af_6328992Dormiani.pdf

Internet

http://celljournal.org/library/upload/article/af_6328992Dormiani.pdf

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