Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pichia pastoris: effect on secretion, activity and thermo-stability

Abstract Methylotrophic yeasts have widely been used as model organisms for understanding cellular functions and biochemical activities in lower eukaryotes. The gene encoding an aspartic protease (MCAP) from Mucor circinelloides DSM 2183 was cloned and expressed into Pichia pastoris using both the n...

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Bibliographic Details
Main Authors: Martin Kangwa, Jose Antonio Gama Salgado, Hector Marcelo Fernandez-Lahore
Format: Article
Language:English
Published: SpringerOpen 2018-10-01
Series:AMB Express
Subjects:
Aspartic proteinase
Mucor circinelloides
Secretion signal peptide
Pichia pastoris
milk-clotting unit (MCU)
N-glycosylation
Online Access:http://link.springer.com/article/10.1186/s13568-018-0691-3

Internet

http://link.springer.com/article/10.1186/s13568-018-0691-3

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