Metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of ET-26-HCl, a new analogue of etomidate
ET-26-HCl, a novel anaesthetic agent with promising pharmacological properties, lacks extensive studies on pharmacokinetics and disposition in vitro and in vivo. In this study, we investigated the metabolic stability, metabolite production and plasma protein binding (PPB) of ET-26-HCl along with its...
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2020-02-01
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doaj-2c459c4a8e324e6db4471c831b2d14212020-11-25T03:52:37ZengThe Royal SocietyRoyal Society Open Science2054-57032020-02-017210.1098/rsos.191666191666Metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of ET-26-HCl, a new analogue of etomidateLu YuXu ChenWen Sheng ZhangLiang ZhengWen Wen XuMing Yu XuXue Hua JiangLing WangET-26-HCl, a novel anaesthetic agent with promising pharmacological properties, lacks extensive studies on pharmacokinetics and disposition in vitro and in vivo. In this study, we investigated the metabolic stability, metabolite production and plasma protein binding (PPB) of ET-26-HCl along with its tissue distribution, excretion and pharmacokinetics in animals after intravenous administration. Ultra-high performance liquid chromatography–tandem quadrupole time-of-flight mass spectrometry identified a total of eight new metabolites after ET-26-HCl biotransformation in liver microsomes from different species. A hypothetical cytochrome P450-metabolic pathway including dehydrogenation, hydroxylation and demethylation was proposed. The PPB rate was highest in mouse and lowest in human. After intravenous administration, ET-26-HCl distributed rapidly to all tissues in rats and beagle dogs, with the highest concentrations in fat and liver. High concentrations of ET-26-acid, a major hydroxylation metabolite of ET-26-HCl, were found in liver, plasma and kidney. Almost complete clearance of ET-26-HCl from plasma occurred within 4 h after administration. Only a small fraction of the parent compound and its acid form were excreted via the urine and faeces. Taken together, the results added to a better understanding of the metabolic and pharmacokinetic properties of ET-26-HCl, which may contribute to the further development of this drug.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191666et-26-hclmetabolitesplasma protein bindingpharmacokineticstissue distributionexcretion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lu Yu Xu Chen Wen Sheng Zhang Liang Zheng Wen Wen Xu Ming Yu Xu Xue Hua Jiang Ling Wang |
spellingShingle |
Lu Yu Xu Chen Wen Sheng Zhang Liang Zheng Wen Wen Xu Ming Yu Xu Xue Hua Jiang Ling Wang Metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of ET-26-HCl, a new analogue of etomidate Royal Society Open Science et-26-hcl metabolites plasma protein binding pharmacokinetics tissue distribution excretion |
author_facet |
Lu Yu Xu Chen Wen Sheng Zhang Liang Zheng Wen Wen Xu Ming Yu Xu Xue Hua Jiang Ling Wang |
author_sort |
Lu Yu |
title |
Metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of ET-26-HCl, a new analogue of etomidate |
title_short |
Metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of ET-26-HCl, a new analogue of etomidate |
title_full |
Metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of ET-26-HCl, a new analogue of etomidate |
title_fullStr |
Metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of ET-26-HCl, a new analogue of etomidate |
title_full_unstemmed |
Metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of ET-26-HCl, a new analogue of etomidate |
title_sort |
metabolite identification, tissue distribution, excretion and preclinical pharmacokinetic studies of et-26-hcl, a new analogue of etomidate |
publisher |
The Royal Society |
series |
Royal Society Open Science |
issn |
2054-5703 |
publishDate |
2020-02-01 |
description |
ET-26-HCl, a novel anaesthetic agent with promising pharmacological properties, lacks extensive studies on pharmacokinetics and disposition in vitro and in vivo. In this study, we investigated the metabolic stability, metabolite production and plasma protein binding (PPB) of ET-26-HCl along with its tissue distribution, excretion and pharmacokinetics in animals after intravenous administration. Ultra-high performance liquid chromatography–tandem quadrupole time-of-flight mass spectrometry identified a total of eight new metabolites after ET-26-HCl biotransformation in liver microsomes from different species. A hypothetical cytochrome P450-metabolic pathway including dehydrogenation, hydroxylation and demethylation was proposed. The PPB rate was highest in mouse and lowest in human. After intravenous administration, ET-26-HCl distributed rapidly to all tissues in rats and beagle dogs, with the highest concentrations in fat and liver. High concentrations of ET-26-acid, a major hydroxylation metabolite of ET-26-HCl, were found in liver, plasma and kidney. Almost complete clearance of ET-26-HCl from plasma occurred within 4 h after administration. Only a small fraction of the parent compound and its acid form were excreted via the urine and faeces. Taken together, the results added to a better understanding of the metabolic and pharmacokinetic properties of ET-26-HCl, which may contribute to the further development of this drug. |
topic |
et-26-hcl metabolites plasma protein binding pharmacokinetics tissue distribution excretion |
url |
https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.191666 |
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