Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Abstract Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism. As a screening tool on shake flask level, the membrane-based fed-batc...

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Bibliographic Details
Main Authors: P. Philip, D. Kern, J. Goldmanns, F. Seiler, A. Schulte, T. Habicher, J. Büchs
Format: Article
Language:English
Published: BMC 2018-05-01
Series:Microbial Cell Factories
Subjects:
Fed-batch
Shake flask
pH
Osmolality
MOPS buffer
Secondary substrate limitation
Online Access:http://link.springer.com/article/10.1186/s12934-018-0917-8

Internet

http://link.springer.com/article/10.1186/s12934-018-0917-8

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