Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask
Abstract Background Screening in the fed-batch operation mode is essential for biological cultivations facing challenges as oxygen limitation, osmotic inhibition, catabolite repression, substrate inhibition or overflow metabolism. As a screening tool on shake flask level, the membrane-based fed-batc...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2018-05-01
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Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12934-018-0917-8 |