Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients

Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients

Abstract Circulating cell-free DNA (cfDNA) has been investigated as a screening tool for many diseases. To avoid expensive and time-consuming DNA isolation, direct quantification PCR assays can be established. However, rigorous validation is required to provide reliable data in the clinical and non-...

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Bibliographic Details
Main Authors: Elmo W. I. Neuberger, Alexandra Brahmer, Tobias Ehlert, Katrin Kluge, Keito F. A. Philippi, Simone C. Boedecker, Julia Weinmann-Menke, Perikles Simon
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-92826-4

Internet

https://doi.org/10.1038/s41598-021-92826-4

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