Quantification of protein cargo loading into engineered extracellular vesicles at single‐vesicle and single‐molecule resolution

Quantification of protein cargo loading into engineered extracellular vesicles at single‐vesicle and single‐molecule resolution

Abstract Extracellular Vesicles (EVs) have been intensively explored for therapeutic delivery of proteins. However, methods to quantify cargo proteins loaded into engineered EVs are lacking. Here, we describe a workflow for EV analysis at the single‐vesicle and single‐molecule level to accurately qu...

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Bibliographic Details
Main Authors: Andreia M. Silva, Elisa Lázaro‐Ibáñez, Anders Gunnarsson, Aditya Dhande, George Daaboul, Ben Peacock, Xabier Osteikoetxea, Nikki Salmond, Kristina Pagh Friis, Olga Shatnyeva, Niek Dekker
Format: Article
Language:English
Published: Taylor & Francis Group 2021-08-01
Series:Journal of Extracellular Vesicles
Subjects:
EV cargo sorting
exosomes
ExoView
extracellular vesicles
nanoflow cytometry
protein delivery vehicle
Online Access:https://doi.org/10.1002/jev2.12130

Internet

https://doi.org/10.1002/jev2.12130

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