Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
The current FDA-approved whole blood stabilization method for circulating tumor cell (CTC) isolation suffers from RNA degradation. Here the authors combine hypothermic preservation and antiplatelet strategies to stabilize whole blood up to 72 h without compromising CTC yield and RNA integrity.
Bibliographic Details
| Main Authors: |
Keith H. K. Wong,
Shannon N. Tessier,
David T. Miyamoto,
Kathleen L. Miller,
Lauren D. Bookstaver,
Thomas R. Carey,
Cleo J. Stannard,
Vishal Thapar,
Eric C. Tai,
Kevin D. Vo,
Erin S. Emmons,
Haley M. Pleskow,
Rebecca D. Sandlin,
Lecia V. Sequist,
David T. Ting,
Daniel A. Haber,
Shyamala Maheswaran,
Shannon L. Stott,
Mehmet Toner |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Publishing Group
2017-11-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-017-01705-y
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