Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
The current FDA-approved whole blood stabilization method for circulating tumor cell (CTC) isolation suffers from RNA degradation. Here the authors combine hypothermic preservation and antiplatelet strategies to stabilize whole blood up to 72 h without compromising CTC yield and RNA integrity.
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| Format: | Article |
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Nature Publishing Group
2017-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-017-01705-y |
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doaj-2cb65508990f455f91b7d509541cd82f2021-05-11T07:14:16ZengNature Publishing GroupNature Communications2041-17232017-11-018111110.1038/s41467-017-01705-yWhole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cellsKeith H. K. Wong0Shannon N. Tessier1David T. Miyamoto2Kathleen L. Miller3Lauren D. Bookstaver4Thomas R. Carey5Cleo J. Stannard6Vishal Thapar7Eric C. Tai8Kevin D. Vo9Erin S. Emmons10Haley M. Pleskow11Rebecca D. Sandlin12Lecia V. Sequist13David T. Ting14Daniel A. Haber15Shyamala Maheswaran16Shannon L. Stott17Mehmet Toner18BioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolBioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolCancer Center, Massachusetts General Hospital, Harvard Medical SchoolBioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolBioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolBioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolBioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolCancer Center, Massachusetts General Hospital, Harvard Medical SchoolCancer Center, Massachusetts General Hospital, Harvard Medical SchoolCancer Center, Massachusetts General Hospital, Harvard Medical SchoolCancer Center, Massachusetts General Hospital, Harvard Medical SchoolDepartment of Radiation Oncology, Massachusetts General Hospital, Harvard Medical SchoolBioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolCancer Center, Massachusetts General Hospital, Harvard Medical SchoolCancer Center, Massachusetts General Hospital, Harvard Medical SchoolCancer Center, Massachusetts General Hospital, Harvard Medical SchoolDepartment of Surgery, Massachusetts General Hospital, Harvard Medical SchoolBioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolBioMEMS Resource Center, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical SchoolThe current FDA-approved whole blood stabilization method for circulating tumor cell (CTC) isolation suffers from RNA degradation. Here the authors combine hypothermic preservation and antiplatelet strategies to stabilize whole blood up to 72 h without compromising CTC yield and RNA integrity.https://doi.org/10.1038/s41467-017-01705-y |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Keith H. K. Wong Shannon N. Tessier David T. Miyamoto Kathleen L. Miller Lauren D. Bookstaver Thomas R. Carey Cleo J. Stannard Vishal Thapar Eric C. Tai Kevin D. Vo Erin S. Emmons Haley M. Pleskow Rebecca D. Sandlin Lecia V. Sequist David T. Ting Daniel A. Haber Shyamala Maheswaran Shannon L. Stott Mehmet Toner |
| spellingShingle |
Keith H. K. Wong Shannon N. Tessier David T. Miyamoto Kathleen L. Miller Lauren D. Bookstaver Thomas R. Carey Cleo J. Stannard Vishal Thapar Eric C. Tai Kevin D. Vo Erin S. Emmons Haley M. Pleskow Rebecca D. Sandlin Lecia V. Sequist David T. Ting Daniel A. Haber Shyamala Maheswaran Shannon L. Stott Mehmet Toner Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells Nature Communications |
| author_facet |
Keith H. K. Wong Shannon N. Tessier David T. Miyamoto Kathleen L. Miller Lauren D. Bookstaver Thomas R. Carey Cleo J. Stannard Vishal Thapar Eric C. Tai Kevin D. Vo Erin S. Emmons Haley M. Pleskow Rebecca D. Sandlin Lecia V. Sequist David T. Ting Daniel A. Haber Shyamala Maheswaran Shannon L. Stott Mehmet Toner |
| author_sort |
Keith H. K. Wong |
| title |
Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells |
| title_short |
Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells |
| title_full |
Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells |
| title_fullStr |
Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells |
| title_full_unstemmed |
Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells |
| title_sort |
whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2017-11-01 |
| description |
The current FDA-approved whole blood stabilization method for circulating tumor cell (CTC) isolation suffers from RNA degradation. Here the authors combine hypothermic preservation and antiplatelet strategies to stabilize whole blood up to 72 h without compromising CTC yield and RNA integrity. |
| url |
https://doi.org/10.1038/s41467-017-01705-y |
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