IL-27-induced gene expression is downregulated in HIV-infected subjects.
OBJECTIVE: To characterize the effect of HIV infection on IL-27-induced gene expression. DESIGN: During HIV infection, cytokine expression and function become deregulated. IL-27 is an important modulator of inflammatory responses. Interestingly, IL-27 can inhibit HIV replication in T cells and monoc...
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doaj-2cdc16ff83a1430c89e0594af40d28c92020-11-24T22:02:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4570610.1371/journal.pone.0045706IL-27-induced gene expression is downregulated in HIV-infected subjects.Christina GuzzoWilma M HopmanNor Fazila Che MatWendy WobeserKatrina GeeOBJECTIVE: To characterize the effect of HIV infection on IL-27-induced gene expression. DESIGN: During HIV infection, cytokine expression and function become deregulated. IL-27 is an important modulator of inflammatory responses. Interestingly, IL-27 can inhibit HIV replication in T cells and monocytes, implicating IL-27 as a potential adjunct to anti-viral treatment. Our previous work demonstrated that circulating HIV may suppress IL-27 expression, therefore, this study, in continuation of our previous work, aimed to understand how HIV affects expression levels of the IL-27 receptor and downstream functions of IL-27. METHODS: Peripheral blood mononuclear cells (PBMC) were isolated from whole blood of HIV negative and HIV positive (viremic) individuals to assess IL-27-induced gene expression by flow cytometry and ELISA. PBMC were also processed for monocyte enrichment to assess IL-27 receptor expression by flow cytometry and real-time PCR. RESULTS: Expression of the IL-27 receptor subunit, gp130, was upregulated in response to IL-27 in HIV negative individuals, however, in HIV positive individuals, this IL-27 response was diminished. Furthermore, we observed downregulation of IL-27-induced IL-6, TNF-α, and IL-10 expression in HIV positive subjects. CONCLUSION: In HIV infection, IL-27-induced gene expression was impaired, indicating HIV-mediated dysregulation of IL-27 functions occurs during HIV infection. This study provides evidence for new viral pathogenic mechanisms contributing to the widespread impairment of immune responses observed in HIV pathogenesis.http://europepmc.org/articles/PMC3458084?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christina Guzzo Wilma M Hopman Nor Fazila Che Mat Wendy Wobeser Katrina Gee |
spellingShingle |
Christina Guzzo Wilma M Hopman Nor Fazila Che Mat Wendy Wobeser Katrina Gee IL-27-induced gene expression is downregulated in HIV-infected subjects. PLoS ONE |
author_facet |
Christina Guzzo Wilma M Hopman Nor Fazila Che Mat Wendy Wobeser Katrina Gee |
author_sort |
Christina Guzzo |
title |
IL-27-induced gene expression is downregulated in HIV-infected subjects. |
title_short |
IL-27-induced gene expression is downregulated in HIV-infected subjects. |
title_full |
IL-27-induced gene expression is downregulated in HIV-infected subjects. |
title_fullStr |
IL-27-induced gene expression is downregulated in HIV-infected subjects. |
title_full_unstemmed |
IL-27-induced gene expression is downregulated in HIV-infected subjects. |
title_sort |
il-27-induced gene expression is downregulated in hiv-infected subjects. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
OBJECTIVE: To characterize the effect of HIV infection on IL-27-induced gene expression. DESIGN: During HIV infection, cytokine expression and function become deregulated. IL-27 is an important modulator of inflammatory responses. Interestingly, IL-27 can inhibit HIV replication in T cells and monocytes, implicating IL-27 as a potential adjunct to anti-viral treatment. Our previous work demonstrated that circulating HIV may suppress IL-27 expression, therefore, this study, in continuation of our previous work, aimed to understand how HIV affects expression levels of the IL-27 receptor and downstream functions of IL-27. METHODS: Peripheral blood mononuclear cells (PBMC) were isolated from whole blood of HIV negative and HIV positive (viremic) individuals to assess IL-27-induced gene expression by flow cytometry and ELISA. PBMC were also processed for monocyte enrichment to assess IL-27 receptor expression by flow cytometry and real-time PCR. RESULTS: Expression of the IL-27 receptor subunit, gp130, was upregulated in response to IL-27 in HIV negative individuals, however, in HIV positive individuals, this IL-27 response was diminished. Furthermore, we observed downregulation of IL-27-induced IL-6, TNF-α, and IL-10 expression in HIV positive subjects. CONCLUSION: In HIV infection, IL-27-induced gene expression was impaired, indicating HIV-mediated dysregulation of IL-27 functions occurs during HIV infection. This study provides evidence for new viral pathogenic mechanisms contributing to the widespread impairment of immune responses observed in HIV pathogenesis. |
url |
http://europepmc.org/articles/PMC3458084?pdf=render |
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