Optimization of PCR–RFLP Directly from the Skin and Nails in Cases of Dermatophy-tosis, Targeting the ITS and the 18S Ribosomal DNA Regions

Purpose: A pan fungal primer targeting the Internal Transcribed Spacer (ITS) region and optimization of PCR-RFLP using a dermatophyte specific primer targeted the 18S ribosomal DNA (rDNA) region were performed for the identification of dermatophyte species and strains directly from clinical speci...

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Bibliographic Details
Main Authors: Elangovan Elavarashi, Anupma Jyoti Kindo, Jagannathan Kalyani
Format: Article
Language:English
Published: JCDR Research and Publications Private Limited 2014-04-01
Series:Journal of Clinical and Diagnostic Research
Subjects:
pcr
Online Access:https://jcdr.net/articles/PDF/2873/10-%205363_E(C)_PF1(M)_F(P)_PF1(P)_PFA(P)_OLF_u.pdf