Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA

ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specif...

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Bibliographic Details
Main Authors: Na Feng, Yazhou Zhou, Yanxiao Fan, Yujing Bi, Ruifu Yang, Yusen Zhou, Xiaoyi Wang
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia
Series:Brazilian Journal of Microbiology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100128&lng=en&tlng=en