Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA
ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specif...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Microbiologia
|
Series: | Brazilian Journal of Microbiology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100128&lng=en&tlng=en |