Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified α-linolenic acid in unanesthetized rats
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), long-chain n-3 PUFAs important for brain and heart function, can be obtained from dietary fish products or by liver synthesis from α-linolenic acid (α-LNA). Their daily human dietary requirements are not clear, and their liver synthesis rat...
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Language: | English |
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Elsevier
2009-04-01
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Series: | Journal of Lipid Research |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520308737 |
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doaj-2d18c2c1f0a543ccaf344c8327277a24 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fei Gao Dale Kiesewetter Lisa Chang Kaizong Ma Jane M. Bell Stanley I. Rapoport Miki Igarashi |
spellingShingle |
Fei Gao Dale Kiesewetter Lisa Chang Kaizong Ma Jane M. Bell Stanley I. Rapoport Miki Igarashi Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified α-linolenic acid in unanesthetized rats Journal of Lipid Research docosahexaenoic acid stable isotopes liver esterified rat kinetics |
author_facet |
Fei Gao Dale Kiesewetter Lisa Chang Kaizong Ma Jane M. Bell Stanley I. Rapoport Miki Igarashi |
author_sort |
Fei Gao |
title |
Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified α-linolenic acid in unanesthetized rats |
title_short |
Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified α-linolenic acid in unanesthetized rats |
title_full |
Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified α-linolenic acid in unanesthetized rats |
title_fullStr |
Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified α-linolenic acid in unanesthetized rats |
title_full_unstemmed |
Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified α-linolenic acid in unanesthetized rats |
title_sort |
whole-body synthesis-secretion rates of long-chain n-3 pufas from circulating unesterified α-linolenic acid in unanesthetized rats |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2009-04-01 |
description |
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), long-chain n-3 PUFAs important for brain and heart function, can be obtained from dietary fish products or by liver synthesis from α-linolenic acid (α-LNA). Their daily human dietary requirements are not clear, and their liver synthesis rates in humans and nonhumans are unknown. We estimated whole-body (presumably liver) synthesis rates in unanesthetized rats by infusing [U-13C]α-LNA intravenously for 2 h and measuring labeled and unlabeled n-3 PUFA in arterial plasma using negative chemical ionization GC-MS. Newly synthesized esterified [13C]DHA, [13C]EPA, and [13C]docosapentaenoic acid (DPA) appeared in arterial plasma after 60 min of infusion, then their concentrations rose in an S-shaped manner. Esterified concentration × plasma volume data were fit with a sigmoidal equation, whose peak first derivatives provided synthesis rates of unlabeled EPA, DPA, and DHA equal to 8.40, 6.27, and 9.84 μmol/day, respectively. The DHA synthesis rate exceeded the published daily rat brain DHA consumption rate by 30-fold, suggesting that liver synthesis from α-LNA could maintain brain DHA homeostasis were DHA absent from the diet. This stable isotope infusion method could be used to quantify whole-body DHA synthesis rates in human subjects. |
topic |
docosahexaenoic acid stable isotopes liver esterified rat kinetics |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520308737 |
work_keys_str_mv |
AT feigao wholebodysynthesissecretionratesoflongchainn3pufasfromcirculatingunesterifiedalinolenicacidinunanesthetizedrats AT dalekiesewetter wholebodysynthesissecretionratesoflongchainn3pufasfromcirculatingunesterifiedalinolenicacidinunanesthetizedrats AT lisachang wholebodysynthesissecretionratesoflongchainn3pufasfromcirculatingunesterifiedalinolenicacidinunanesthetizedrats AT kaizongma wholebodysynthesissecretionratesoflongchainn3pufasfromcirculatingunesterifiedalinolenicacidinunanesthetizedrats AT janembell wholebodysynthesissecretionratesoflongchainn3pufasfromcirculatingunesterifiedalinolenicacidinunanesthetizedrats AT stanleyirapoport wholebodysynthesissecretionratesoflongchainn3pufasfromcirculatingunesterifiedalinolenicacidinunanesthetizedrats AT mikiigarashi wholebodysynthesissecretionratesoflongchainn3pufasfromcirculatingunesterifiedalinolenicacidinunanesthetizedrats |
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spelling |
doaj-2d18c2c1f0a543ccaf344c8327277a242021-04-28T05:57:08ZengElsevierJournal of Lipid Research0022-22752009-04-01504749758Whole-body synthesis-secretion rates of long-chain n-3 PUFAs from circulating unesterified α-linolenic acid in unanesthetized ratsFei Gao0Dale Kiesewetter1Lisa Chang2Kaizong Ma3Jane M. Bell4Stanley I. Rapoport5Miki Igarashi6Brain Physiology and Metabolism Section, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892; Positron Emission Tomography Radiochemistry Group, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892Brain Physiology and Metabolism Section, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892; Positron Emission Tomography Radiochemistry Group, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892Brain Physiology and Metabolism Section, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892; Positron Emission Tomography Radiochemistry Group, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892Brain Physiology and Metabolism Section, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892; Positron Emission Tomography Radiochemistry Group, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892Brain Physiology and Metabolism Section, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892; Positron Emission Tomography Radiochemistry Group, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892Brain Physiology and Metabolism Section, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892; Positron Emission Tomography Radiochemistry Group, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892Brain Physiology and Metabolism Section, National Institute on Aging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892; Positron Emission Tomography Radiochemistry Group, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD 20892Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), long-chain n-3 PUFAs important for brain and heart function, can be obtained from dietary fish products or by liver synthesis from α-linolenic acid (α-LNA). Their daily human dietary requirements are not clear, and their liver synthesis rates in humans and nonhumans are unknown. We estimated whole-body (presumably liver) synthesis rates in unanesthetized rats by infusing [U-13C]α-LNA intravenously for 2 h and measuring labeled and unlabeled n-3 PUFA in arterial plasma using negative chemical ionization GC-MS. Newly synthesized esterified [13C]DHA, [13C]EPA, and [13C]docosapentaenoic acid (DPA) appeared in arterial plasma after 60 min of infusion, then their concentrations rose in an S-shaped manner. Esterified concentration × plasma volume data were fit with a sigmoidal equation, whose peak first derivatives provided synthesis rates of unlabeled EPA, DPA, and DHA equal to 8.40, 6.27, and 9.84 μmol/day, respectively. The DHA synthesis rate exceeded the published daily rat brain DHA consumption rate by 30-fold, suggesting that liver synthesis from α-LNA could maintain brain DHA homeostasis were DHA absent from the diet. This stable isotope infusion method could be used to quantify whole-body DHA synthesis rates in human subjects.http://www.sciencedirect.com/science/article/pii/S0022227520308737docosahexaenoic acidstable isotopesliveresterifiedratkinetics |