A Modified Technique for Culturing Primary Fetal Rat Cortical Neurons

The study explored a modified primary culture system for fetal rat cortical neurons. Day E18 embryos from pregnant Sprague Dawley rats were microdissected under a stereoscope. To minimize enzymatic damage to the cultured neurons, we applied a sequential digestion protocol using papain and Dnase I. T...

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Bibliographic Details
Main Authors: Sui-Yi Xu, Yong-Min Wu, Zhong Ji, Xiao-Ya Gao, Su-Yue Pan
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2012/803930