A map of protein dynamics during cell-cycle progression and cell-cycle exit.

The cell-cycle field has identified the core regulators that drive the cell cycle, but we do not have a clear map of the dynamics of these regulators during cell-cycle progression versus cell-cycle exit. Here we use single-cell time-lapse microscopy of Cyclin-Dependent Kinase 2 (CDK2) activity follo...

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Main Authors:	Sara Gookin, Mingwei Min, Harsha Phadke, Mingyu Chung, Justin Moser, Iain Miller, Dylan Carter, Sabrina L Spencer
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2017-09-01
Series:	PLoS Biology
Online Access:	http://europepmc.org/articles/PMC5608403?pdf=render

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spelling	doaj-2dd7841c8d704303af2b8059aa29fc1c2021-07-02T03:59:59ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852017-09-01159e200326810.1371/journal.pbio.2003268A map of protein dynamics during cell-cycle progression and cell-cycle exit.Sara GookinMingwei MinHarsha PhadkeMingyu ChungJustin MoserIain MillerDylan CarterSabrina L SpencerThe cell-cycle field has identified the core regulators that drive the cell cycle, but we do not have a clear map of the dynamics of these regulators during cell-cycle progression versus cell-cycle exit. Here we use single-cell time-lapse microscopy of Cyclin-Dependent Kinase 2 (CDK2) activity followed by endpoint immunofluorescence and computational cell synchronization to determine the temporal dynamics of key cell-cycle proteins in asynchronously cycling human cells. We identify several unexpected patterns for core cell-cycle proteins in actively proliferating (CDK2-increasing) versus spontaneously quiescent (CDK2-low) cells, including Cyclin D1, the levels of which we find to be higher in spontaneously quiescent versus proliferating cells. We also identify proteins with concentrations that steadily increase or decrease the longer cells are in quiescence, suggesting the existence of a continuum of quiescence depths. Our single-cell measurements thus provide a rich resource for the field by characterizing protein dynamics during proliferation versus quiescence.http://europepmc.org/articles/PMC5608403?pdf=render
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sara Gookin Mingwei Min Harsha Phadke Mingyu Chung Justin Moser Iain Miller Dylan Carter Sabrina L Spencer
spellingShingle	Sara Gookin Mingwei Min Harsha Phadke Mingyu Chung Justin Moser Iain Miller Dylan Carter Sabrina L Spencer A map of protein dynamics during cell-cycle progression and cell-cycle exit. PLoS Biology
author_facet	Sara Gookin Mingwei Min Harsha Phadke Mingyu Chung Justin Moser Iain Miller Dylan Carter Sabrina L Spencer
author_sort	Sara Gookin
title	A map of protein dynamics during cell-cycle progression and cell-cycle exit.
title_short	A map of protein dynamics during cell-cycle progression and cell-cycle exit.
title_full	A map of protein dynamics during cell-cycle progression and cell-cycle exit.
title_fullStr	A map of protein dynamics during cell-cycle progression and cell-cycle exit.
title_full_unstemmed	A map of protein dynamics during cell-cycle progression and cell-cycle exit.
title_sort	map of protein dynamics during cell-cycle progression and cell-cycle exit.
publisher	Public Library of Science (PLoS)
series	PLoS Biology
issn	1544-9173 1545-7885
publishDate	2017-09-01
description	The cell-cycle field has identified the core regulators that drive the cell cycle, but we do not have a clear map of the dynamics of these regulators during cell-cycle progression versus cell-cycle exit. Here we use single-cell time-lapse microscopy of Cyclin-Dependent Kinase 2 (CDK2) activity followed by endpoint immunofluorescence and computational cell synchronization to determine the temporal dynamics of key cell-cycle proteins in asynchronously cycling human cells. We identify several unexpected patterns for core cell-cycle proteins in actively proliferating (CDK2-increasing) versus spontaneously quiescent (CDK2-low) cells, including Cyclin D1, the levels of which we find to be higher in spontaneously quiescent versus proliferating cells. We also identify proteins with concentrations that steadily increase or decrease the longer cells are in quiescence, suggesting the existence of a continuum of quiescence depths. Our single-cell measurements thus provide a rich resource for the field by characterizing protein dynamics during proliferation versus quiescence.
url	http://europepmc.org/articles/PMC5608403?pdf=render
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A map of protein dynamics during cell-cycle progression and cell-cycle exit.

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