Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris

Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris

Abstract Background Glycosylphosphatidylinositol (GPI)-anchored glycoproteins have diverse intrinsic functions in yeasts, and they also have different uses in vitro. In this study, the functions of potential GPI proteins in Pichia pastoris were explored by gene knockout approaches. Results Through a...

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Bibliographic Details
Main Authors: Pan Wang, Ying Lin, Chengjuan Zou, Fengguang Zhao, Shuli Liang, Suiping Zheng, Shuangyan Han
Format: Article
Language:English
Published: BMC 2020-08-01
Series:BMC Microbiology
Subjects:
GPI protein
Deletion
Phenotypic screen
Pichia pastoris
Online Access:http://link.springer.com/article/10.1186/s12866-020-01928-y

Internet

http://link.springer.com/article/10.1186/s12866-020-01928-y

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